Ternary glycerol-based deep eutectic solvents: Physicochemical properties and enzymatic activity

The present study investigates deep eutectic solvents (DESs) as potential media for enzymatic hydrolysis. A series of ternary ammonium and phosphonium-based DESs were prepared at different molar ratios by mixing with aqueous glycerol (85%). physicochemical properties including surface tension, conductivity, density, viscosity measured a temperature range 298.15 K – 363.15 K. points highly influenced the variation temperature. point choline chloride: glycerol: water (ratio 1: 2.55: 2.28) methyltriphenylphosphonium bromide:glycerol:water 4.25: 3.75) is 213.4 255.8 K, respectively. stability lipase enzyme isolated from porcine pancreas (PPL) Rhizopus niveus (RNL) toward hydrolysis in medium was investigated. PPL showed higher activity compared to RNL DESs. Molecular docking simulation selected DES substrate (p-nitrophenyl palmitate) also reported. It worth noting that systems would be viable activators reactions.